Coding

Part:BBa_K2663003:Design

Designed by: Elizabeth Johnson   Group: iGEM18_WashU_StLouis   (2018-10-17)


AvrSr35 Avirulence Factor from Wheat Stem Rust Fungus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1910
    Illegal BamHI site found at 2037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9
    Illegal SapI site found at 1210


Design Notes

The sequence was codon optimized for Saccharomyces cerevisiae EBY100.


Source

The sequence of this part comes from Puccinia graminis f. sp. tritici.

References